2a receptor antibody  (Santa Cruz Biotechnology)

Journal: Drug Design, Development and Therapy

Article Title: Cordycepin Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Inhibiting IL-6/IL-6R-Mediated p38 MAPK and NF-κB Activation Through Adenosine A 2A Receptor Stimulation

doi: 10.2147/DDDT.S575035

Figure Lengend Snippet: Cordycepin abates DSS-induced damage in NCM460 cells by activating adenosine receptor A 2A . ( A ) The cAMP levels in NCM460 cells under treatment with 2% DSS and/or 1 μmol/L and 10 μmol/L COR. ( B ) The cAMP levels in NCM460 cells under treatment with 2% DSS, 10 μmol/L COR, 5 μmol/L SCH58261 (SCH), and / or 5 μmol/L DPCPX for 24 h. The release levels of ( C ) LDH, ( D ) IL-1β, ( E ) IL-6, and ( F ) TNF-α were measured by using Elisa. ( G ) Representative images of PI staining and the percentage of PI-positive cells, scar bar = 100 μm. ( H – J ) The protein expression of ZO-1, A 2A AR and A 1 AR in NCM460 cells was determined by using Western blotting assay. The results were representative of three independent experiments and expressed as mean ± SEM. Data were compared using two-tailed Student’s t tests. vs. control group; # P < 0.05, vs. control group; *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the 2% DSS group.

Article Snippet: Adenosine A 2A receptor Antibody (A 2A AR, sc-32261) was purchased from Santa Cruz Biotechnology (USA).

Techniques: Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Two Tailed Test, Control

Journal: Drug Design, Development and Therapy

Article Title: Cordycepin Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Inhibiting IL-6/IL-6R-Mediated p38 MAPK and NF-κB Activation Through Adenosine A 2A Receptor Stimulation

doi: 10.2147/DDDT.S575035

Figure Lengend Snippet: The results of molecular docking and molecular dynamics simulation of adenosine receptor A 2A -cordycepin. ( A ) 2D (left) and 3D (right) molecular docking diagrams of A 2A AR-COR. The PDB ID of the A 2A AR protein is 5nm4. The yellow stick represents the structure of COR, and the cartoon depicts the protein structure. The green sticks indicate amino acid residues within 4 Å that form hydrogen bonds with COR. Yellow dashed lines represent hydrogen bonds, with adjacent numbers denoting the distance between COR and the hydrogen-bonded amino acids. White sticks represent amino acids involved in hydrophobic interactions with COR. ( B ) The Root Mean Square Deviation (RMSD). ( C ) The number of hydrogen bonds of the complex. ( D ) The Root Mean Square Fluctuation (RMSF) values for the complex. ( E ) The Radius of Gyration (Rg) values of the complex. ( F ) Solvent Accessible Surface Area (SASA) of the complex.

Article Snippet: Adenosine A 2A receptor Antibody (A 2A AR, sc-32261) was purchased from Santa Cruz Biotechnology (USA).

Techniques: Solvent

Journal: Drug Design, Development and Therapy

Article Title: Cordycepin Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Inhibiting IL-6/IL-6R-Mediated p38 MAPK and NF-κB Activation Through Adenosine A 2A Receptor Stimulation

doi: 10.2147/DDDT.S575035

Figure Lengend Snippet: Cordycepin inhibits IL-6/IL-6R-mediated p38 MAPK and NF-κB phosphorylation activation relying on activation of adenosine receptor A 2A . ( A – E ) Representative blot bands and relative expression levels for IL-6R, NF-κB P-p65, NF-κB p65, NF-κB P-p65/p65, detected by Western blotting. ( F – I ) Representative blot bands and relative expression levels for P-p38 MAPK, p38 MAPK, P-p38/p38 MAPK. ( J – M ) Representative blot bands and relative expression levels for P-STAT3, STAT3, P-STAT3/STAT3. Data were presented as the means ± SEM of three mice in each group and were compared using two-tailed Student’s t tests. # P < 0.05, ## P < 0.01 vs. control group; *P < 0.05, **P < 0.01 vs. the DSS model (DSS) group.

Article Snippet: Adenosine A 2A receptor Antibody (A 2A AR, sc-32261) was purchased from Santa Cruz Biotechnology (USA).

Techniques: Phospho-proteomics, Activation Assay, Expressing, Western Blot, Two Tailed Test, Control

Journal: Drug Design, Development and Therapy

Article Title: Cordycepin Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice by Inhibiting IL-6/IL-6R-Mediated p38 MAPK and NF-κB Activation Through Adenosine A 2A Receptor Stimulation

doi: 10.2147/DDDT.S575035

Figure Lengend Snippet: The ameliorative effect of cordycepin on DSS-induced colitis is attributed to its activation of A 2A AR, which inhibits IL-6/IL-6R-mediated phosphorylation activation of p38 MAPK and NF-κB.

Article Snippet: Adenosine A 2A receptor Antibody (A 2A AR, sc-32261) was purchased from Santa Cruz Biotechnology (USA).

Techniques: Activation Assay, Phospho-proteomics